Table 4. Kinetics parameters of the interactions of chimeric constructs with mCSF-1 or with mIL-34 measured by QCM.

Constructs	mCSF-1a				mIL-34b
	ka (106.M−1.s−1)	kd (10−4.s−1)	KD (nM)	ka (104.M−1.s−1)		kd* (10−4.s−1)	KD (nM)
mCD115 (WT)	2.1 ± 0.01	< 1	< < 1	5.0 ± 1.00		19.0 ± 0.03	38 ± 8.0
2/3D1 mutD2	1.6 ± 0.01	< 1	< < 1	5.1 ± 0.60		16.0 ± 0.01	31 ± 3.0
hCD115 (WT)	NB	NB	NB	29.0 ± 0.20		3.1 ± 0.09	1 ± 0.0
1/3D1	1.0 ± 0.02	< 1	< < 1	2.7 ± 2.00		11.0 ± 0.02	41 ± 3.0
2/3D1	1.4 ± 0.01	< 1	< < 1	2.5 ± 2.00		9.6 ± 0.20	38 ± 3.0
hD1	1.6 ± 0.01	< 1	< < 1	0.8 ± 0.04		2.9 ± 0.10	37 ± 2.0

^a Binding of mCSF-1 to CD115 constructs. Deduced from curves presented in Figure 8B and Figure S5A. ^bBinding of mIL-34 to CD115 constructs. Deduced from curves presented in Figure 8C and Figure S5B. *Error considered as 0.01 when error from the fitting model was found below 0.01. NB: No detectable binding