Fig. 2.

Design of PCR analyses used to detect allelic drop out in carriers of the MSH2 inversion. a Long and short PCR amplicons and their positions on chromosome 2 relative to the 3′ inversion breakpoint, MSH2 intron 8, and SNP rs7607076 are depicted. b Sequencing results of rs7607076 in the long and short amplicons for patients tested for the MSH-2 inversion by use of inversion-specific PCR. Only one allele is amplified in the long PCR of inversion carriers which results in the SNP appearing to be homozygous