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. Author manuscript; available in PMC: 2014 Sep 24.
Published in final edited form as: Nat Commun. 2014 Mar 24;5:3483. doi: 10.1038/ncomms4483

Figure 3. Several UPRmt inducing RNAi clones shorten lifespan.

Figure 3

(a) N2 fed EV(RNAi) (mean 14.8 ± .1 days, n = 335), N2 fed tomm-22(RNAi) (mean 12.7 ± .2 days, n = 327, p < 0.0001). (b) N2 fed EV(RNAi) (mean 16.3 ± .2 days, n = 260), N2 fed T09B4.9(RNAi) (mean 14.5 ± .2 days, n = 289, p < 0.0001). (c) N2 fed EV(RNAi) (mean 16.7 ± .1 days, n = 293), N2 fed dnj-21(RNAi) (mean 14.9 ± .2 days, n = 270, p < 0.0001). (d) N2 fed EV(RNAi) (mean 16.5 ± .2 days, n = 279), N2 fed F45G2.8(RNAi) (mean 15.2 ± .2 days, n = 291, p < 0.0001). (e) N2 fed EV(RNAi) (mean 16.3 ± .2 days, n = 260), N2 fed E04A4.5(RNAi) (mean 15.1 ± .2 days, n = 199, p < 0.0001). (f) N2 fed EV(RNAi) (mean 16.3 ± .2 days, n = 260), N2 fed F15D3.7(RNAi) (mean 15.1 ± .2 days, n = 217, p < 0.0001). (g) The hsp-6p::gfp reporter is induced by knockdown of RNAi clones that reduce lifespan. hsp-6p::gfp worms were placed onto RNAi bacteria from egg and grown at 25°C. GFP measurements were taken three days later. Scale bar, 0.3 mm. Lifespan experiments in this figure were performed at 25°C. N=3 independent experiments, with pooled data shown. Lifespans are indicated as mean +/− SEM and p-values were calculated using Wilcoxon rank-sum test. Data by individual experiment and statistical analysis provided in Supplementary Materials (Supplementary Data 1).