Fig. 1.

Preferred natural amino acid substrates for LTA4H. Initial screening of the 19-membered natural amino acid substrate library and 18 d-amino acid substrates. Enzyme activity was monitored using an fMax multi-well fluorescence plate reader (Molecular Devices) at excitation wavelength of 355 nm and an emission wavelength of 460 nm. The x-axis represents the abbreviated amino acid names (for full name and structure see Fig. S1). The y-axis represents the average relative activity expressed as a percent of the best amino acid substrate. All structures and information about fluorogenic substrates are in Online Resource 1