Table I.
Primer and probe sequences for real-time PCR of HPV-16 and HPV-18 L1 and E7 DNA and HPV-16 and HPV-18 E7 mRNA
| Sequence (5'→3') | Nucleotide position | |
|---|---|---|
| DNA | ||
| HPV-16-E7-forward primer | AGAACCGGACAGAGCCCATTAC | 696-717 |
| HPV-16-E7-reverse primer | GCCCATTAACAGGTCTTCCAAAG | 794-816 |
| HPV-16-E7-probe | CGCACAACCGAAGCGTAGAGTCACACTT | 739-766 |
| HPV-16-L1-forward primer | TCCAGATACACAGCGGCTGG | 5971-5990 |
| HPV-16-L1-reverse primer | AATAAAGGATGGCCACTAATGCCC | 6040-6063 |
| HPV-16-L1-probe | AATGGCTGACCACGACCTACCTCAACA | 6007-6033 |
| HPV-18-E7-forward primer | GAACCACAACGTCACACAATG | 752-772 |
| HPV-18-E7-reverse primer | CAGAAACAGCTGCTGGAATG | 843-862 |
| HPV-18-E7-probe | TCTGCTGAGCTTTCTACTACTAGCTCAATTCTG | 799-831 |
| HPV-18-L1-forward primer | TTGGTTCAGGCTGGATTGC | 7020-7038 |
| HPV-18-L1-reverse primer | GCTTGGCAGGTTTAGAAGAC | 7091-7110 |
| HPV-18-L1 probe | CCTCGCAAACGTTCTGCTCCATCTGCCAC | 7059-7087 |
| mRNA | ||
| HPV-16-E7-forward primer | CCGGACAGAGCCCATTACAAT | 700-720 |
| HPV-16-E7-reverse primer | ACGTGTGTGCTTTGTACGCAC | 782-762 |
| HPV-16-E7-probe | TGTTGCAAGTGTGACTCTACGCTTCGGT | 733-760 |
| HPV-18-E7-forward primer | GACTCAGAGGAAGAAAACGATGAAA | 686-710 |
| HPV-18-E7-reverse primer | GTGACGTTGTGGTTCGGCT | 766-748 |
| HPV-18-E7-probe | TGGAGTTAATCATCAACATTTACCA | 715-739 |