Figure 5.

Immunofluorescent localization of creatine kinase (CK) and phosphotyrosine in human sperm. (A) Nonstained; (B) CK stained; (C) phosphotyrosine stained; (D-F) CK-phosphotyrosine (green-red) double-stained sperm; (G) proportion of double immunostaining of phosphotyrosine and CK in HA-bound and initial sperm fractions. Data represent the mean proportion of each pattern for the 13 patients. Two hundred sperm were examined for each patient (2600 sperm in all). Asterisks indicate significant differences (P < .05).