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. Author manuscript; available in PMC: 2014 Oct 17.
Published in final edited form as: Cell Rep. 2013 Oct 3;5(1):194–206. doi: 10.1016/j.celrep.2013.08.040

Figure 1. Ku70 Interacts with TRF1 and TRF2 at Telomeres.

Figure 1

(A) Fluorescence quantification of PCA for Ku70 or Rad21 with either TRF1 or TRF2. Shown are the averages for three independent transfections. Error bars represent SD of the mean.

(B) Colocalization of PCA for Ku70 with TRF1 or TRF2, YFP channel, with the DsRed-TRF2 telomeric marker, RFP channel, and (merge) in HEK293T cells.

(C) Fluorescence quantification of PCA for Ku70 and TRF1 or Ku70 and TRF2 with coexpression of either FLAG-tagged empty vector (EV), TRF1 or TRF2, and TRF1ΔAΔM or TRF2ΔBΔM. p values were determined by Student’s two-tailed unpaired t test with *, p < 0.05; **, p < 0.01; and ns, not significant. For V[1]-TRF1/V[2]-Ku70: p = 0.0345 for EV versus TRF2ΔBΔ Mand p = 0.0011 for TRF2 versus TRF2ΔBΔM. For V[1]-TRF2/V[2]-Ku70: p = 0.8566 for EV versus TRF1ΔAΔM and p = 0.0343 for TRF1 versus TRF1ΔAΔM. The error bars represent SD of the mean.

(D) Colocalization of PCA for Ku70 with TRF1 with coexpression of either FLAG-EV or FLAG-TRF2ΔBΔM (merge) in HEK293T cells.

See also Figure S1.