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. Author manuscript; available in PMC: 2014 Apr 12.
Published in final edited form as: Gynecol Oncol. 2008 May 29;110(2):237–245. doi: 10.1016/j.ygyno.2008.04.013

Fig. 1. S1P affects expression, secretion and activation of gelatinases.

Fig. 1

DOV13 cells were plated and serum starved, as described in methods, and treated with the indicated S1P concentrations in SFM for 6 hours before CM was collected and (A) applied to the DQ gelatinase activity assay; and (B) separated on 15% SDS-PAGE zymography gels and stained. (C) Cell were treated as above for 4 or 24hrs, then cell surface proteins were biotin labeled and isolated from cell extracts through affinity streptavidin binding and separated on gradient 12.5% SDS PAGE gels, and analyzed by western analysis. Bars represent average of 2–3 assays, and gelatinolytic activity is represented relative to control (vehicle for S1P). Error bars=SEM. * Indicates p<0.05; ** = p<0.005.