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. 2014 Feb 3;16(5):637–651. doi: 10.1093/neuonc/not300

Fig. 4.

Fig. 4.

Posttranscriptional regulation of LAMB1 by miR-124-5p. (A) A putative miR-124-5p binding site in the 3′ UTR of LAMB1 was mutated by replacing the cytosine with guanine (marked by an asterisk). (B and C) LAMB1 mRNA and protein expression in U87 and U251 cells cotransfected with the wild-type (WT) or mutated (MUT) LAMB1 3′ UTR, along with miR-124-5p mimic, miRNA mimic negative control (NC), miR-124-5p inhibitor, or miRNA inhibitor negative control (NC inhibitor), as determined by quantitative PCR (left) and Western blotting (right). (D and E) Normalized luciferase activity in U87 and U251 cells cotransfected with the WT or MUT LAMB1 3′ UTR, along with miR-124-5p mimic, NC, miR-124-5p inhibitor, or NC inhibitor as determined using the dual-luciferase reporter assay system. Data are shown as mean ± SD. *P < .05; **P < .01.