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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: Eur Heart J. 2012 Jul 26;34(32):2566–2574. doi: 10.1093/eurheartj/ehs227

Figure 3. PPARα-activation modulates intracellular cholesterol trafficking, increases cytoplasmic lipid droplet formation and reduces apoB secretion by Caco-2/TC7.

Figure 3

Caco-2/TC7 were incubated with NBD-cholesterol-micelles (50μg/well) for 4h and then for 4h with non-fluorescent micelles. GW7647 (600nM) was added 24h before and during the NBD-cholesterol-loading period (representative of 3 independent experiments). (A) NBD-fluorescence was quantified using Image J at each optical slice. (B) NBD-cholesterol (green), ZO-1 (red) and Hoechst (blue) were visualized by confocal microscopy. (C) ApoB in the basolateral medium was quantified by ELISA. Values are expressed as means ± SEM. t-test; *P<.05 Vehicle vs treatment.