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. Author manuscript; available in PMC: 2014 Apr 15.
Published in final edited form as: Eur Heart J. 2012 Jul 26;34(32):2566–2574. doi: 10.1093/eurheartj/ehs227

Figure 4. PPARα-activation decreases cholesterol esterification in Caco-2/TC7.

Figure 4

Caco-2/TC7 were incubated with [3H]-cholesterol-micelles (7.5μCi/well) for 48h. GW7647 (600nM) was added 24h before and during the [3H]-cholesterol-loading. Intracellular lipids (A) and lipids secreted in the basolateral compartment (B) were extracted and separated by TLC. Results are expressed as means of CE percent of total cholesterol (±SEM). Q-PCR analysis of ACAT-2 (C) was performed on GW7647 (600nM)-treated Caco-2/TC7 for 24h. Values are expressed as means ± SD. t-test; *P<.05; **P<.01 Vehicle vs treatment.