Table 1.

Bacterial strains, plasmids, and primers

Bacterial strains
Species	Strain name	Reference
S. pneumoniae	EF3030	Briles et al., 1992
	EF3030 luxS−	this study
M. catarrhalis	O35E	Unhanand, et al., 1992
	O35E bro−	Balder, et al., 2013

Plasmids
Name	Reference
pCR2.1	Invitrogen®
pSpR	Whitby et al., 1998
pCRSpLuxSSp	this study

Primers
Name	Sequence	Reference
Splux		this study
Forward	5′-CGTGTTCGTCGCAATCCATACTC-3′
Reverse	5′-ACAAGAAATCATCCGCCGTTACTA-3′
Spluxver		this study
Forward	5′-GACGTTCAAAGGCATCATCTG-3′
Reverse	5′-CCTACTGCCGGCCTTCACACTATC-3′
M13		Invitrogen ®
Forward (−20)	5′-GTAAAACGACGGCCAGT-3′
Reverse	5′-CAGGAAACAGCTATGAC-3′