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. Author manuscript; available in PMC: 2015 Feb 4.
Published in final edited form as: Curr Opin Struct Biol. 2014 Feb 4;0:115–124. doi: 10.1016/j.sbi.2014.01.008

Figure 2. Crystal structures of Tetrahymena p65 xRRM, Teb1 A, B, and C, and Takifugu rubripes TRBD.

Figure 2

(a) p65-TER interactions. At top, schematic of domain structure of p65. (left) Secondary structure schematic of Tetrahymena TER with Stem-Loop 4 bulge guanosine (cyan) adenosine (orange) colored (center) p65 xRRM (green) free (PDB ID: 4EYT) and in complex with Stem 4 (PDB ID: 4ERD). The unstructured α3x in the free xRRM is shown as a dashed dark green line, and is structured in the complex with Stem 4 (magenta). (b) Teb1 domains and interaction with telomere DNA. At top, schematic of domain structure of Teb1. Crystal structures of (left) Teb1A from Teb1AB co-crystal structure (blue) with telomeric DNA (grey) (PDB ID: 3U58), (middle) Teb1B (yellow) (PDB ID: 3U4Z), and (right) Teb1C (orange) with zinc (silver) coordinated by four cysteines of the zinc ribbon (PDB ID: 3U50). (c) Takifugu rubripes TERT TRBD structure. At top, schematic of domain structure of Takifugu rubripes TERT. (left)Takifugu rubripes (PDB ID: 4LMO) and (right) Tetrahymena TRBD (PDB ID: 2R4G) crystal structures. T-motif (magenta) and CP (orange) form the T-CP pocket, which is elongated in the Takifugu rubripes TRBD by the TFLY (green) motif. Vertebrate-specific RNA binding motif (red) and homologous UV active residues (orange, green, and yellow) are all located in a similar location.