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MRM (433.3→ 126.1) chromatogram of derivatized pregnanolone (A) DMS off (transparent mode) (B) COV= 7V, SV=3700 (C) COV=11 V, SV=3700 and (D) MRM chromatogram of mixture of allopregnanolone, pregnanolone, epiallopregnanolone and epipregnanolone at COV=7 V, SV=3700: trans- (a) and cis- (b) allopregnanolone, trans-pregnanolone (c), trans- (e) and cis- (f) epiallopregnanolone, trans- (g) and cis- (h) epipregnanolone.
