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. Author manuscript; available in PMC: 2015 Apr 12.
Published in final edited form as: Anal Bioanal Chem. 2013 Oct 12;405(29):9497–9508. doi: 10.1007/s00216-013-7391-2

Table 1.

Calibration curve and QC intra- and inter- day accuracy and precision of derivatized allopregnanolone and pregnanolone without DMS

Compound MRM transition Calibration curve 10-25,000 pg/mL
Slope Intercept r 2
Allopregnanolone 433.3→374.4 0.34483 0.00109 0.9977
433.3→126.1 0.05140 0.00017 0.9985
Pregnanolone 433.3→374.4 Interference
433.3→126.1 0.05078 0.00030 0.9994
QC intra-day accuracy (%) (n=4)
LQC
( 50 pg/mL)		 MQC
( 500 pg/mL)
 HQC
( 5000 pg/mL)
Allopregnanolone 433.3→374.4 108.3 ±2.8 106.3 ±2.4 97.3 ±3.0
433.3→126.1 106.1 ±1.1 104.4 ±1.4 97.9 ±3.0
Pregnanolone 433.3→374.4 Interference
433.3→126.1 103.2 ±3.3 104.3 ±1.3 98.9 ±1.3
QC inter-day accuracy (%) (n=8)
LQC
( 50 pg/mL)		 MQC
( 500 pg/mL)		 HQC
( 5000 pg/mL)
Allopregnanolone 433.3→374.4 106.9 ±3.6 105.2 ±2.7 96.8 ±2.3
433.3→126.1 107.3 ±2.6 102.9 ±2.3 97.4 ±2.3
Pregnanolone 433.3→374.4 Interference
433.3→126.1 104.0 ±2.4 100.6 ±3.1 96.9 ±2.8