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. Author manuscript; available in PMC: 2015 Apr 12.
Published in final edited form as: Anal Bioanal Chem. 2013 Oct 12;405(29):9497–9508. doi: 10.1007/s00216-013-7391-2

Table 2.

Calibration curve and QC intra- and inter- day accuracy and precision of derivatized allopregnanolone and pregnanolone with DMS

Compound MRM transition Calibration curve 10-25,000 pg/mL
Slope Intercept r 2
Allopregnanolone 433.3→374.4 0.27881 0.00096 0.9980
433.3→126.1 0.05460 0.00015 0.9969
Pregnanolone 433.3→374.4 0.31453 0.00158 0.9965
433.3→126.1 0.03995 0.00019 0.9940
QC intra-day accuracy (%) (n=4)
LQC
( 50 pg/mL)		 MQC
( 500 pg/mL)		 HQC
( 5000 pg/mL)
Allopregnanolone 433.3→374.4 105.0 ±1.9 103.5 ±1.3 99.4 ±0.3
433.3→126.1 105.0 ±2.8 103.3 ±1.3 98.9 ±1.3
Pregnanolone 433.3→374.4 106.6 ±2.5 104.4 ±1.1 98.7 ±1.8
433.3→126.1 106.4 ±2.5 104.6 ±1.3 99.4 ±1.4
QC inter-day accuracy (%) (n=8)
LQC
( 50 pg/mL)		 MQC
( 500 pg/mL)		 HQC
( 5000 pg/mL)
Allopregnanolone 433.3→374.4 105.5 ±1.7 103.4 ±1.4 99.4 ±0.5
433.3→126.1 104.7 ±2.6 103.3 ±1.0 98.3 ±1.0
Pregnanolone 433.3→374.4 106.0 ±2.8 104.6 ±1.6 98.0 ±1.5
433.3→126.1 105.1 ±4.1 103.5 ±2.6 99.2 ±1.1