Skip to main content
. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Eur J Immunol. 2014 Jan 20;44(4):970–982. doi: 10.1002/eji.201344276

Figure 3. Similar Ca2+ responses by FO B cells expressing different levels of sIgM upon equivalent sIgM engagement.

Figure 3

(A) Representative staining of FO B cells (B220pos CD23hi) pre-labeled with low (red, 0.5 μg/ml), intermediate (blue, 2 μg/ml), or high (green, 5 μg/ml) concentrations of Bio-Fab b7-6 following addition of DyLight 649 conjugated streptavidin (1 μg/ml). (B) Residual unoccupied sIgM on B cells within the defined fluorescence (gated population) was detected with DyLight 488-Fab b7-6. (C) Ca2+ traces of B cells expressing the defined fluorescence following addition of DyLight 649 conjugated streptavidin. Data are representative of 3 independent experiments with similar results.