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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Eur J Immunol. 2014 Jan 20;44(4):970–982. doi: 10.1002/eji.201344276

Figure 4. In vivo antibody production by FO IgMlo B cells.

Figure 4

Total IgG1κ on day 14 following transfer of FACS purified mature FO IgMlo or IgMhi B cells into A/J κ−/− recipients. (A) Transfer of 2.5 × 105 FO IgMlo or IgMhi B cells and immunization with 50 μg of GαMκ in alum. (B) Transfer of 5 × 104 mature FO IgMlo or IgMhi B cells and immunization with 50 μg of Protein L in alum. In both experiments, mature FO B cells were defined as B220pos CD23hi CD24int, and the IgMlo and IgMhi populations constituted the lowest and highest 10% of the FO B cell population respectively. Data are representative of 2-3 independent transfer experiments.