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. Author manuscript; available in PMC: 2014 Apr 13.
Published in final edited form as: Nat Med. 2012 May;18(5):791–798. doi: 10.1038/nm.2717

Figure 4.

Figure 4

Cleaved caspase-1 p10 colocalizes with activated macrophages in CEP-MSA–immunized mice. (a–c) Immunostaining of retinal cryosections of CEP-MSA–immunized mice showing localization of F4/80+ macrophages to regions of the choroid (a), extending from the choroid toward the Bruch’s membrane (b) and present above the RPE in the outer segments (OS) and outer nuclear layer (ONL) of the retina (c). Differential contrast images are shown at the top in a and b and on the left in c, and fluorescent images (with F4/80 in red and DAPI in blue) are shown at the bottom in a and b and on the right in c. (d,e) Colabeling of retinal cryosections of CEP-MSA–immunized mice, with caspase-1 p10 (red) and F4/80 (green) showing colocalization in a macrophage present within and transcending the choroid and the Bruch’s membrane (d) and a macrophage protrusion in the outer segment of the retina (e). (f) Colabeling of retinal cryosections of CEP-MSA–immunized mice showing colocalization of NLRP3 (red) and F4/80 (green). (g) A higher magnification of the NLRP3 and F4/80 staining in f.