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. 2014 Mar 11;13:54. doi: 10.1186/1476-4598-13-54

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Copyright © 2014 Aesoy et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Arnt activates transcription from the CDKN2B promoter. A-C) U2OS cells were transfected in 12-well plates with the reporter gene construct -35CDKN2B/Luc (300 ng) and expression plasmids encoding Miz-1 (Miz1/pcDNA) or wt Arnt (Arnt/Flag/pCMV) at the indicated concentrations (A), wt Arnt or mutated variants of Arnt/Flag (Arnt (T115D/S126A)/Flag/pCMV denoted “2xmut” and Arnt (L114A/T115D/R118G/S126A) /Flag/pCMV denoted “4xmut” (100 ng each) (B), and wt Arnt and wt (Myc/pcDNA3) or a mutated variant of Myc (V394D) (Myc (V394D)/pcDNA3) (100 ng each) as indicated (C). The luciferase activity is presented as average +/− stdev, n = 9 in A; n = 6 in B; n = 12 in C. Statistical analyses: (A)Student’s T-test, (B)ANOVA (Bonferroni), (C)ANOVA (Dunnett’s T3); ***p ≤ 0.001, ns: non significant.