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. 2014 Jan 25;14:7. doi: 10.1186/1475-2867-14-7

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Copyright © 2014 LI et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Clonogenic assay. A and B described the cell survival condition after irradiation. X-ray irradiation was performed as planed in 6 Gy/fraction and 2 Gy/fraction with total dose 60 Gy. An appropriate number of cells in different generations were seed in 6-well plates. Each group was irradiated at the dose of 0 Gy, 1 Gy, 2 Gy, 4 Gy, 6 Gy, 8 Gy, 10 Gy, respectively. After incubation for 14 days, the colonies were fixed and stained by crystal violet. The coloniesâ€‰>â€‰50 cells were scored as viable colonies. The data was analyzed by single-hit multi-target model and survival curve of each group was described by Graphpad prism 5.0 software.