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. Author manuscript; available in PMC: 2014 Apr 13.
Published in final edited form as: J Cancer Sci Ther. 2013 Jan 21;5(2):58–65.

Figure 2.

Figure 2

Figure 2

Figure 2

Figure 2

Figure 2

Figure 2a: SETD4 is overexpressed in ER-negative breast cancer. SETD4 mRNA expression was evaluated by qPCR. The SETD4 expression values for the HCC-1954, MCF-7, CAMA-1, SKBR-3, MDA-MB 231, MDA-MB 436 and MDA-MB 468 cells were normalized to that of the HCC-1954-BL control cells.

Figure 2b: The expression of SETD4 in MDA-MB 231 and MCF-7 cells was normalized relative to normal, non-cancerous cells. β-actin and RPLS27a were used as endogenous controls.

Figure 2c: Immunoblot analysis of total protein extracts of breast cancer cell lines. Densitometric analysis showed that SETD4 is up-regulated in MACL-1, MGSO-3, MDA-MB 231 cells (ER-negative) and down-regulated in MCF-7 cells (ER-positive).

Figure 2d: Immunoblots were performed to quantify the expression of SETD4 in breast cancer tissues.

Figure 2e: Densitometric analysis confirmed that triple-negative samples showed elevated expression of SETD4 compared with tumors that were positive for ER, PR and HER2. Means followed by the same letter indicate no significant difference by Tukey’s test (p<0.001).