Figure 1.

The expression of miR-210 and HIF-1α was assayed by qRT-PCR and western plot on hypoxia and normoxia in C28/I2 human chondrocytes. C28/I2 cells were expose to hypoxia or normoxia at 12 h, 24 h, 48 h, and 72 h. Then, the HIF-1α mRNA was detected by qRT-PCR in cells (a). The expression of miR-210 on hypoxia was contrasted with that on normoxia (c). Expression of HIF-1α protein was also confirmed by western plot at 12 h, 24 h, 48 h, and 72 h in hypoxic C28/I2 cells (b). Five individual experiments were conducted. Student's t-test was performed to statistically analyzed. The significant differences were denoted as *P < 0.05 and **P < 0.01.