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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Mol Microbiol. 2014 Jan 21;91(5):965–975. doi: 10.1111/mmi.12508

Figure 2.

Figure 2

In vivo stability of the AcrAB-TolC complex. The His-tagged wild type and mutant AcrB proteins were extracted from envelopes by DDM and affinity purified as described in the Experimental procedures section. Fractions containing the AcrBHis protein were analyzed by SDS-PAGE and the presence of AcrA, AcrBHis, and TolC were determined by Western blot analysis using antibodies specific to these proteins. Prior to SDS-PAGE analysis, protein samples in a SDS buffer were either boiled (uneven lanes) or kept at room temperature (even lanes).