Skip to main content
. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Mol Microbiol. 2014 Jan 21;91(5):965–975. doi: 10.1111/mmi.12508

Table 3.

Minimal inhibitory concentration profiles of strains expressing various AcrA and AcrB proteins.

AcrAB proteinsa AcrA mutant isolation methodb Minimal inhibitory concentration (μg ml-1)
Erythromycin Novobiocin SDS
AcrA+ AcrB+ NA >128 >128 >800
AcrA- AcrB- NA 2 2 25
AcrA+ AcrB H1Δ252-256 SDM 16 8-16 100
AcrAE43K AcrB H1Δ252-256 Spontaneous >128 128 400
AcrAV44I AcrB H1Δ252-256 Spontaneous >128 128 200
AcrAS195P AcrB H1Δ252-256 Spontaneous >128 128 400
AcrAL222Q AcrB H1Δ252-256 Spontaneous >128 128 400
AcrAL222R AcrB H1Δ252-256 Spontaneous >128 128 400
AcrAΔ222-224 AcrB H1Δ252-256 Spontaneous >128 128 400
AcrAT224S AcrB H1Δ252-256 Spontaneous >128 128 400
AcrAF230S AcrB H1Δ252-256 Spontaneous >128 128 200-400
AcrAG248E AcrB H1Δ252-256 Spontaneous >128 128 400
AcrAG249C AcrB H1Δ252-256 Spontaneous >128 128 400
AcrAL222Q AcrB+ SDM 128 128 ND
AcrAG248E AcrB+ SDM >128 128 ND
AcrAS83G AcrB H1Δ252-256 SDM 128 64 400
AcrAT111P AcrB H1Δ252-256 SDM 128 64 400
AcrAA135T AcrB H1Δ252-256 SDM 128 64 400
AcrAN146T AcrB H1Δ252-256 SDM 128 64 400
a

AcrAB proteins were expressed from pACYC184 in a ΔacrAB background.

NA, not applicable; SDM, site-directed mutagenesis; ND, not determined.