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. Author manuscript; available in PMC: 2014 Apr 13.
Published in final edited form as: Dev Cell. 2013 Mar 25;24(6):600–611. doi: 10.1016/j.devcel.2013.02.011

Figure 3. Tracing splanchnopleural mesoderm derivatives using CFDA-SE.

Figure 3

24h after CFDA-SE labeling. Same section triple stained with DAPI (A), CFDA-SE (B) and CD45 (C). The dotted line indicates the limit between the endothelium and the sub-aortic mesenchyme.

DAPI staining reveals the topography of the tissues. CFDA-SE stains the sub-aortic mesenchyme and the coelomic epithelium. CD45 stains the aortic clusters. (D) merge of DAPI, CFDA-SE and CD45 signals. The aortic HC clusters were never green in keeping with an endothelial origin of HC clusters and the complementary roles of aortic endothelium and sub-aortic mesenchyme in aortic hematopoiesis. Bar=80μm.