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Experimental assay and data analysis. (A) Human embryonic NSCs were loaded into microfluidic devices mounted on chamber slides, resulting in random cell seeding. The migratory path of each individual cell within a stable CXCL12 gradient was tracked for up to 17 hrs. (B) The migration speed (D/time) and chemotactic index (X/D) were calculated for each cell track. (C) CXCL12 and/or BDNF did not significantly increase NSC proliferation upon 48-hr exposure (p > 0.05).
