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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Integr Biol (Camb). 2014 Feb;6(2):127–142. doi: 10.1039/c3ib40188j

Figure 5.

Figure 5

A) Percentage of encapsulated organoids per well that demonstrated contractile behavior and B) total organoid count per well when cultured within either submerged or air-liquid interface cultures under severely hypoxic (1%) or normoxic (20%) conditions for two weeks. Organoids were encapsulated within matrices of type-I collagen densities ranging from 1.8 to 2.7 mg/mL in the presence or absence of exogenous R-spondin1 (R-spo1). Statistical significance of data comparing cultures with and without exogenous R-spo1 was analyzed by ANOVA and Kruskal-Wallis test (p<0.01, n.s.= not significant) and is displayed as mean ± standard error of the mean. A minimum of 300 organoids were analyzed per condition.