Fig. 4.

All the behavior tests were performed on day 2, 4, 7, 14, 21, and 28 after the surgery. All data are presented as means±S.E. from 7 and 12 survived animals from the sham-operated and MCAo animals, respectively. (A) The MCAo animals remained on the rotarod for shorter time compared to the sham-operated animals. (B) The MCAo animals showed the biased turning to the right side when reaching the corner, whereas the sham operated animals did not show any directional biasness. (C, D) The latency to turn downward and reach the floor from the top of the pole was increased in the MCAo group compared to the sham group. (E) Data are presented from the survived animals (●) or from total animals (▲) including the 8 dead animals. The maximum score of 5 was given to the dead animals. Either way, MCAo animals showed severe deficits than the sham animals (○). (F) The loss of body weight was higher in the MCAo group compared to the sham (t-test, ^*p<0.05, ^**p<0.01).