Fig. 1.
The effect of melittin on inflammation in the lungs of hSOD1G93A mice. Melittin (0.1 µg/g) was injected subcutaneously bilaterally three times for a week in 14-week-old hSOD1G93A transgenic mice. Representative data from independent Western blot experiments of Iba-1 (A) and CD14 (B) in the lung of hSOD1G93A transgenic mice (n=4~5 per group). Immunoblotting of TNF-α (C) and COX2 (D) proteins from the lungs of melittin- or saline-treated hSOD1G93A transgenic mice. Tubulin is used as a loading control of total protein. Data are shown as the mean±SEM. Data were analyzed with PRISM analysis and statistical significance was calculated by t-test. *p<0.05, compared to saline-treated hSOD1G93A transgenic mice. MT-: saline-treated hSOD1G93A mice, MT+: melittin-treated hSOD1G93A mice.