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. 2014 Apr 7;8:20. doi: 10.3389/fnana.2014.00020

FIGURE 5.

FIGURE 5

Expressions of layer-specific marker genes in the turtle and chick pallia at the mid-neurogenetic stage. Coronal sections of E18 turtle (A,C,E,G,I,K) and E7 chick (B,D,F,H,J,L) pallia, in which the mediolateral axis runs left to right and the dorsoventral axis runs top to bottom. (A,B) HE staining of the pallium. (C) Tbr1 expression exhibits a medial-low, lateral-high gradient across the whole pallium of the turtle. (D) Tbr1 expression covers the whole chick pallium except for the marginal and VZs. (E,F) Er81 is not expressed in the turtle pallium (E), but is expressed specifically in the medial part of the chick pallium (F). The inset is an enlargement of the rectangular box in the medial region. (G,H) Fezf2 expression exhibits a medial-high, lateral-low gradient in the VZ and differentiated neurons in both species. (IL) Two layer 2/3 (L2/3) marker genes, Mef2c and Satb2, are expressed in the lateral region of the turtle and chick pallia. Scale bars: 250 μm.