Table 1. Corrected programs of PCR.
Corrected program | PCR program | Target gene | Bacteria |
94-40 s | 95-10 min | lacZ | Coliform bacteria |
94-1 min | 55-1 min | ||
55-40 s | 72-1 min | ||
72-30 s | 72-2 min | ||
Cycles 23 | Cycle 25 | ||
94-30 s | 95-10 min | uidA | E. coli |
94-45 s | |||
55-30 s | 62-45 s | ||
72-45 s | |||
72-30 s | 72-2 min | ||
Cycles 23 | Cycles 30 |