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. Author manuscript; available in PMC: 2015 Mar 15.
Published in final edited form as: J Immunol. 2014 Feb 19;192(6):2837–2845. doi: 10.4049/jimmunol.1302484

Figure 3. CC-induced release of cytokines from PBMC and granulocytes.

Figure 3

Human PBMC and granulocytes were isolated from whole blood and resuspended in plasma/PBS before incubation with CC (3 × 107 particles/ml) for 5.5 hours. CC-induced production of the cytokines (A) IL-1β, (B) TNF, (C) IL-6, (D) IL-8, (E) MIP-1α, and (F) MCP-1 from PBMC and granulocytes were quantified by multiplex analysis and compared (*, p <0.05). (G) Myeloperoxidase (MPO) from PBMC and granulocytes was detected by ELISA (*, p <0.05). Data plotted are mean ± SD in triplicate determinations in one out of six independent experiments.