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. Author manuscript; available in PMC: 2015 Mar 15.
Published in final edited form as: J Immunol. 2014 Feb 19;192(6):2837–2845. doi: 10.4049/jimmunol.1302484

Figure 4. CC-induced CD11b expression and cytokine production in whole blood from a C5-deficient person.

Figure 4

Whole blood from a C5 deficient donor, or C5 deficient donor reconstituted with purified C5 (50 µg/ml), or blood from three healthy donors were incubated with CC (3 × 107 particles/ml), PBS/HSA or LPS at 37°C. (A) TCC detected by ELISA in plasma after 30 minutes. (B, C) Median fluorescent intensity (MFI) of CD11b on granulocytes and monocytes measured after 15 minutes. Plasma collected after 6 hours incubation was analyzed by multiplex for (D) IL-1β, (E) TNF, (F) IL-6, and (G) IL-8. Mean of triplicate determinations are shown for the C5 defect donor samples, and mean of three control donors are shown. Error bars: ±SD.