Whole blood from a C5 deficient donor, or C5 deficient donor reconstituted with purified C5 (50 µg/ml), or blood from three healthy donors were incubated with CC (3 × 107 particles/ml), PBS/HSA or LPS at 37°C. (A) TCC detected by ELISA in plasma after 30 minutes. (B, C) Median fluorescent intensity (MFI) of CD11b on granulocytes and monocytes measured after 15 minutes. Plasma collected after 6 hours incubation was analyzed by multiplex for (D) IL-1β, (E) TNF, (F) IL-6, and (G) IL-8. Mean of triplicate determinations are shown for the C5 defect donor samples, and mean of three control donors are shown. Error bars: ±SD.