Table 1.

Comparison of EC50 values of known compounds dispensed by the HP D300 instrument with traditional pin tool dispensing in three assays. 95% confidence intervals for EC50 values are shown in parentheses.

Compound	HP D300 - without DMSO backfill	HP D300 - with DMSO backfill	Pin tool – 1536-well
α-galactosidase enzyme assay
Lansoprazole	1.20×10−5 (1.07–1.34×10−5)	1.15×10−5 (0.99–1.32×10−5)	8.95×10−6 (7.40–10.8×10−6)
PMA	1.47×10−7 (1.30–1.60×10−7)	1.67×10−7 (1.47–1.88×10−7)	1.69×10−7 (1.53–1.86×10−7)
DGJ	1.38×10−9 (1.27–1.48×10−9)	1.69×10−7 (1.53–1.86×10−7)	3.64×10−9 (3.36–3.92×10−9)
GR reporter gene assay
Prednisolone	3.74×10−9 (2.90–4.82×10−9)	9.77×10−9 (6.20–1.53×10−9)	1.09×10−8 (8.92–1.33×10−8)
Dexamethasone	3.85×10−10 (2.90–5.09×10−10)	3.11×10−10 (2.71–3.57×10−10)	5.37×10−10 (3.75–7.69×10−10)
Cortisol	6.74×10−9 (5.10–8.90×10−9)	4.37×10−9 (3.49–5.47×10−9)	2.55×10−8 (2.09–3.10×10−8)
Cytotoxicity assay (ATP content)
Tamoxifen	9.00×10−6 (3.60–2.24×10−6)	8.77×10−6 (4.55–1.68×10−6)	3.21×10−5 (1.70–6.04×10−5)
Daunorubicin	2.52×10−7 (2.34–2.70×10−7)	1.89×10−7 (1.73–2.05×10−7)	3.80×10−7 (3.32–4.34×10−7)
TOAB	2.50×10+ (1.81–3.45×10−7)	1.66×10−7 (1.36–2.04×10+)	1.75×10−7 (1.37–2.23×10−7)