Histology and immunohistochemistry of AT and H tumors. In AT tumors, necrosis was found even in small tumors, such as the tumor with V = 118 mm3 (A) for, and increased with tumor volume (V = 2207 mm3) (B). Pimonidazole-positive area also increased with tumor volume of AT tumors (D, E). In contrast, in Dunning H tumors no necrosis was observed even in large tumors (V = 1949 mm3) (C) and only minimal pimonidazole activity was found (F).