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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Dev Dyn. 2013 Sep 6;242(11):1293–1306. doi: 10.1002/dvdy.24025

Figure 6.

Figure 6

Whole mount mRNA in situ hybridization shows Bmp4 and Wnt5a mRNA expression is changed in C251X homozygotic mutants at E11.5 (A-L). A-D: The expression of Shh, and Fgf8 in the wild-type and the mutant do not show obvious differences. E-F: Bmp4 is expressed by the AER and subridge mesenchyme in the wild-type. However, its expression is reduced in the subridge mesenchyme in the mutant (F, triangle). G-H: Wnt5a is profoundly expressed by the outer half of the limb bud, but its expression is greatly reduced in the mutants. I-J: Gremlin1 is expressed in the mesenchyme and there is no obvious expression pattern difference between wild-type and mutant. K-L: Prickle1 is variably expressed in the limb bud. The expression pattern might be the developing skeleton condensates (K). Prickle1 mRNA expression is reduced in the mutants, especially the expression in the anterior limb bud (L). The hand is imaged from the dorsal side, with distal up and posterior to the right. Scale bar is 500 μm.