Figure 3.
Lack of IFN-γ production was correlated with the resistance of FPM1V.EFGFP/8R cells to killing by 4O1/C8. (a) FPM1V.EFGFP or FPM1V.EFGFP/8R cells (5 × 104/well) were mixed with 4O1/C8 cells (5 × 105/well) at an E : T ratio of 10 : 1 in the presence or absence of m8Δ (1 × 105 PFU/well) and subjected to flow-cytometric analysis for the expression of EGFP at the indicated days. Percentage of EGFP positive cells is indicated in each panel. (b) Production of IFN-γ in the supernatants of mixed culture prepared in (a) was measured by ELISA at the indicated days of culture. The data represent the mean ± SD of triplicate wells. Statistical significance was determined as P < 0.001. Similar results were obtained in two independent experiments.