Table 1.
Primer sequences used in the real-time RT-PCR validation of microarray analyses.
| Target gene symbol | Primer sequences (5′-3′) | Target size (bp) | T m (°C) |
|---|---|---|---|
| NR_045555-F | GTTGCAAGGAAACCTTTGGA | 96 | 60 |
| NR_045555-R | CTGCATGCTGTTGACCTTGT | ||
| NR_027621-F | CTGCGTGGATTGCTACAAGA | 102 | 60 |
| NR_027621-R | CCTTCATAGGCCACCACACT | ||
| XR_111050-F | ATGGCCAGTTCGTTTCTCAC | 60 | |
| XR_111050-R | AAGACACGTCCTTGGTTTGG | ||
| NR_037595-F | CCCTGTGCAAGAGCACATAA | 60 | |
| NR_037595-R | TGCCAGCTCATACAAGATGC | ||
| NR_033651-F | CCCCTTGGTATTCTCCCAAT | 60 | |
| NR_033651-R | CAGCCTTTTGTTGGGTGTTT | ||
| NR_037182-F | CTTCTGCAGGAGGAATCCAG | 60 | |
| NR_037182-R | TCCCAGTTTTTGGTGACTCC | ||
| GAPDH-F | CGGACCAATACGACCAAATCCG | 83 | 60 |
| GAPDH-R | AGCCACATCGCTCAGACACC | ||
| HOXA9-F | CGGTTATGGCATTAAACCTGAACCG | 67 | 60 |
| HOXA9-R | GTGAGTGTCAAGCGTGGGACAG | ||
| HOXC8-F | CGGTAAGTTCCAAGGTCTGATACCG | 99 | 60 |
| HOXC8-R | CGTCTCCCAGCCTCATGTTTC | ||
| WNT2B-F | CTTTCCTTTGCACCAGCTTC | 52 | 60 |
| WNT2B-R | TACCCTTCCTCTTGCACACC | ||
| BARX1-F | CGCTTCGAGAAGCAGAAGTA | 111 | 60 |
| BARX1-R | CTTCATCCTCCGATTCTGGT | ||
| IGFBP5-F | GCACCTGAGATGAGACAGGA | 139 | 60 |
| IGFBP5-R | TGTAGAATCCTTTGCGGTCA | ||
| S100A4-F | GTACTTGGTGTCCACCTTCCACAAGTAC | 60 | |
| S100A4-R | CCGGGTCAGCAGCTCCTTTAG |