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. 2014 Winter;13(1):199–206.

Table 1.

Time table of sequences of events in the staining procedure

Sequence of event Stage Material Time
1 Dewaxing of paraffin Toluene 5-6 minutes (if necessary repeated)
2 Hydration Alcohol 100
Alcohol 90
Alcohol 70
Alcohol 50
Distilled water
5 min
5 min
5 min
5 min
5 min
3 Staining Haematoxylin
Washing with water
Alcohol 50
Alcohol 70
Eosin
3-5 min
5 min
5 min
5 min
5 min
4 Dehydration Alcohol 90
Absolute Alcohol
30 second
30 second
5 Clearing Toluene 5-10 minutes