Table 1.
Sequence of event | Stage | Material | Time |
---|---|---|---|
1 | Dewaxing of paraffin | Toluene | 5-6 minutes (if necessary repeated) |
2 | Hydration | Alcohol 100 Alcohol 90 Alcohol 70 Alcohol 50 Distilled water |
5 min 5 min 5 min 5 min 5 min |
3 | Staining | Haematoxylin Washing with water Alcohol 50 Alcohol 70 Eosin |
3-5 min 5 min 5 min 5 min 5 min |
4 | Dehydration | Alcohol 90 Absolute Alcohol |
30 second 30 second |
5 | Clearing | Toluene | 5-10 minutes |