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. Author manuscript; available in PMC: 2015 Mar 27.
Published in final edited form as: Cell Rep. 2014 Mar 13;6(6):1017–1025. doi: 10.1016/j.celrep.2014.01.043

Fig. 1. mVP24 interacts with Keap1 in co-immunoprecipitation assays.

Fig. 1

(A) Co-immunoprecipitations (co-IP) with anti-HA antibody were performed on lysates of HEK293T cells co-transfected with plasmids for Flag-Keap1 and HA-mVP24 or HA-eVP24. Western blots were performed for Flag and HA. (B) Schematic diagram of Flag tagged Keap1 domain deletion mutants used in (C). (C) Flag-Keap1 domain deletion mutant constructs were co-expressed in HEK293T cells with HA-mVP24 and analyzed by co-IP with anti-Flag antibody. (D) HA-mVP24 and either Flag-Keap1 or Flag-Keap1 R415A were analyzed by co-IP as in (C). (E) Overlay of the mVP24 structural model (orange) on the determined eVP24 structure (purple). The mVP24 K-loop (amino acids 205–212) is indicated in red. (F) Flag-Keap1 and HA-mVP24 wild-type or mutants were analyzed by co-IP as in (A) and (C). (G) Flag-Keap1 and HA-mVP24, eVP24, eVP24 DIEPCCGE or eVP24 K-loop were co-expressed in HEK293T cells and analyzed by co-IP as in (A). (H) Flag-mVP24 and HA-Keap1, bat-Keap1 and bat-Kelch were co-expressed in HEK293T cells and analyzed by co-IP as in (C). See also Figure S1.