Figure 6.

Tissue migratory cDC express immune dampening genes in the steady state. Bar graphs show fold changes of (a) immunomodulatory and Cd40 transcripts, (b) inflammatory transcripts in purified lung resident CD103⁺ cDC (gray), Poly: I:C treated lung CD103⁺ cDC (white), LN tissue resident CD8⁺ cDC (black) and lung migratory (Mig) CD103⁺ cDC (red) over the minimum value of the four compared populations. (c) LN single cell suspensions were analyzed by flow cytometry. Histograms show the expression of CD200, FAS, CD40, and PD-L1 in gated DAPI⁻CD45⁺CD11c^hiMHCII^Int resident cDC (blue) and DAPI⁻CD45⁺CD11c^intMHCII^hi migratory cDC (pink) compared to isotype controls (gray). (d) LN resident cDC (left panels) and migratory cDC (right panels) were sorted, cytospun, and stained with secondary mAb alone (top panels) or anti-Pias-3 mAb (bottom panels). Magnification (63×). LuLN: lung draining LN; SDLN: skin draining LN; SI: small intestine, LC: Langerhans Cell. Data shown are representative of three different experiments. *Replicate n ≥3 in graphs unless listed otherwise in Table 1.