Figure 3.

The bilayer resistance response to MelP5 by electrochemical impedance spectroscopy. (A) Aliquots of MelP5 were added to the 60 μM lipid vesicles in the sample chamber to give peptide:lipid ratios of 1:1000, 1:2500, 1:5000, 1:10000, and 1:25000. The resistance of the POPC/cholesterol bilayers was measured before peptide addition, and every 2 min after. Each trace represents an average of at least three independent experiments. The standard deviation of the three measurements is indicated by an error bar at the end. (B) Comparison of the resistance changes caused by melittin and MelP5 at P:L = 1:1000.