Analysis of
cross-linking reactions containing purified His-Ste14p
and different photoactive probes. (A) Fluorescent imaging, (B) immunoblot
analysis with NeutrAvidin HRP, and (C) immunoblot analysis with α-Ste14
. Experiments were performed with 16, 18, and 19. For this experiment, purified His-Ste14p (0.25
μg) was incubated with the probes indicated (50 μM) and
irradiated on ice for 30 min followed by fractionation via SDS-PAGE.
The resulting gel was visualized using (A) a fluorescence scanner
or transferred to a nitrocellulose membrane and visualized using (B)
NeutrAvidin HRP or (C) an α-Ste14 antibody. The data shown is
from one of three replicates of this experiment.