. 2014 Feb 6;79(5):1971–1978. doi: 10.1021/jo402600b

Table 1. In Vitro Reaction Kinetics for a-Factor Peptides Methylated by His-Ste14p.

compound	V_max (pmol min^–1 mg^–1)^a	K_m(app) (μM)^b	V_max/K_m (pmol min^–1 mg^–1 μM^–1)^a^,^b
AFC(22)	870 ± 15	15.9 ± 0.9	54
16	947 ± 4	6.6 ± 0.2	143
17	1919 ± 30	12.2 ± 0.1	157
18(22)	762 ± 28	14.6 ± 0.3	52
19	217 ± 3	27.8 ± 1	7.0

Reported as pmol of methyl groups transferred to substrate.

Because these experiments were performed at a single concentration of SAM and the biphasic nature of the membrane suspension makes the concentration of the peptide substrate unknown (because of partitioning into the lipid membranes), these experiments provide only apparent values for K_m.