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. 2014 Feb 21;136(11):4172–4185. doi: 10.1021/ja4109352

Figure 7.

Figure 7

ChIP analysis of the effect of IMC-76 and IMC-48 given either singly (A and B) or in sequential order (IMC-76 followed by IMC-48) (C) on promoter occupancy of Sp1 and hnRNP LL. For single drug treatments (A and B), two concentrations (0.5 and 2 μM) of IMC-76 or IMC-48 with DMSO as a control were incubated with MCF-7 and BJAB cells, respectively, for 24 h. For sequential treatments (C), antagonism between IMC-76 and IMC-48 was shown through restoration of Sp1 and hnRNP LL promoter occupancy levels following administration of IMC-48 after prior knockdown with IMC-76 in MCF-7 cells. MCF-7 cells were treated with DMSO or 2 μM of IMC-76 for 24 h. In a similar way, other MCF-7 cells were treated first with 2 μM of IMC-76 and then with 2 or 4 μM of IMC-48 for a further 24 h. IP was performed with antibodies to Sp1 and hnRNP LL and IgG as a negative control and acetyl-histone H3 (AcH3) as a positive control. The P values (***P < 0.001, **P < 0.01) were determined by one-way ANOVA analysis.