Skip to main content
. 2014 Feb 14;53(9):1521–1526. doi: 10.1021/bi401556p

Figure 1.

Figure 1

Optimization of AdoMet production catalyzed by AdoMet synthetase. The products of a coupled reaction are shown, where AdoMet synthetase produces AdoMet, which is then used by M.SssI to methylate substrate DNA (linearized pUC19). The concentrations of substrates l-methionine and MgATP were each increased from 0.5 to 6 mM for the 60 min reaction time, followed by restriction digestion with HpaII and gel electrophoresis, shown as a negative image of an ethidium-stained agarose gel, to determine the extent of DNA methylation achieved with each substrate level.