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Efficiency of DNA methylation with AdoMet analogues determined at a fixed level (100 μM) of each substrate at different time intervals at 37 °C. The amount of fully methylated DNA was determined by the total pixel count of the largest band that represents the fully protected pUC19 DNA using AdoMet (◆), AdoMet ethyl ester (▲), or AdoMet methyl ester (■) as the methyl donor.
